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RAS Chemistry & Material ScienceЖурнал аналитической химии Journal of Analytical Chemistry

  • ISSN (Print) 0044-4502
  • ISSN (Online) 3034-512X

Joint determination of nine uremic toxins and choline in blood serum using high-performance liquid chromatography with tandem mass spectrometric detection

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PII
10.31857/S0044450224080094-1
DOI
10.31857/S0044450224080094
Publication type
Article
Status
Published
Authors
T. I. Alyushina
  • Affiliation: Research Institute of Nephrology, I.P. Pavlov First St. Petersburg State Medical University
Volume/ Edition
Volume 79 / Issue number 8
Pages
900-909
Abstract
This paper presents a method for the simultaneous determination of nine uremic toxins and choline in blood serum. The target substances were selected based on literature data as potential biomarkers for assessing the severity and progression of immunoglobulin A nephropathy, a kidney disease that leads to disability, and, without timely treatment, death in young and middle-aged individuals. Ultrafiltration is used to separately determine free and protein-bound indolic uremic toxins in the blood. The use of high-performance liquid chromatography combined with high-resolution tandem mass spectrometry ensures satisfactory accuracy of the analysis without complete chromatographic separation of analytes under standard reversed-phase HPLC conditions. For calibration purposes, an albumin solution in phosphate buffer was used as a surrogate for blood serum. The protein concentration of 45 mg/mL and pH 7.4 match the characteristics of native blood serum. A pilot experiment demonstrated the feasibility of determining key indicators of the gut microbiome’s state—choline and trimethylamine N-oxide (TMAO)—in dried blood spots.
Keywords
уремические токсины холин сыворотка крови иммуноглобулин А нефропатия высокоэффективная жидкостная хроматография с тандемным масс-спектрометрическим детектированием высокого разрешения (ВЭЖХ-МС/МС ВР) триметиламиноксид (ТМАО) сухие пятна крови
Date of publication
15.08.2024
Year of publication
2024
Number of purchasers
0
Views
55

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