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RAS Chemistry & Material ScienceЖурнал аналитической химии Journal of Analytical Chemistry

  • ISSN (Print) 0044-4502
  • ISSN (Online) 3034-512X

PHYTOCHEMICAL ANALYSIS OF EXTRACTS FROM SEVERAL PLANTS GROWING IN RUSSIA USING DEEP EUTECTIC SOLVENTS

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PII
S3034512X25110039-1
DOI
10.7868/S3034512X25110039
Publication type
Article
Status
Published
Authors
T.N. Bochko
  • Affiliation: Saint Petersburg State University, Institute of Chemistry
N.A. Ogrenich
  • Affiliation: Baranovichi Maternity Hospital
M.A. Malyshko
  • Affiliation: 16th City Clinical Polyclinic
A.Yu. Shishov
  • Affiliation: Saint Petersburg State University, Institute of Chemistry
Volume/ Edition
Volume 80 / Issue number 11
Pages
1141-1153
Abstract
Medicinal plants are an important source of biologically active compounds used in medicine for the prevention and treatment of various diseases. The main classes of such compounds—alkaloids, glycosides, flavonoids, essential oils, tannins, polyphenols, and polysaccharides—exhibit a wide range of pharmacological activities (antimicrobial, anti-inflammatory, antiseptic, antioxidant, etc.). Phytochemical screening of plant extracts is essential for identifying these compounds and developing new highly active therapeutic agents. Spectrophotometric phytochemical screening was conducted for alkaloids, tannins, flavonoids, phenolic compounds, steroidal cardiac glycosides, and polysaccharides, along with the determination of antioxidant activity in extracts of three medicinal plants from different families: milky ripe oats (Avena sativa L.), madder root (Rubia tinctorum L.), and common heather (Calluna vulgaris L.). High-performance liquid chromatography was employed for a more detailed analysis of the extracts. Both traditional solvents (methanol, ethanol, water-ethanol mixture, water, acetonitrile) and a new class of solvents—deep eutectic solvents—were used as extractants. Extraction of biologically active compounds was performed using ultrasound at 40–80 °C for 10–60 minutes. Optimal extraction conditions for the main compound groups were established: 80 °C and 60 minutes for traditional solvents; 50°C for 30 minutes (heather) and 60°C for 30 minutes (madder root and oats) when using deep eutectic solvents. Deep eutectic solvents enable the extraction of target bioactive compounds under milder conditions, demonstrating their potential as environmentally friendly extractants in phytochemical studies.
Keywords
биологически активные вещества Array Array Array спектрофотометрия хроматография глубокие эвтектические растворители
Date of publication
01.11.2025
Year of publication
2025
Number of purchasers
0
Views
58

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