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RAS Chemistry & Material ScienceЖурнал аналитической химии Journal of Analytical Chemistry

  • ISSN (Print) 0044-4502
  • ISSN (Online) 3034-512X

CHALLENGES AND FEATURES OF POLYSACCHARIDE VISUALIZATION IN SORBENT LAYERS DURING PLANAR CHROMATOGRAPHY

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PII
S3034512X25110043-1
DOI
10.7868/S3034512X25110043
Publication type
Article
Status
Published
Authors
I.I. Malakhova
  • Affiliation: Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre "Kurchatov Institute" - Institute of Macromolecular Compounds
A.S. Kirillov
  • Affiliation: Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre "Kurchatov Institute" - Institute of Macromolecular Compounds
N.I. Gorshkov
  • Affiliation: Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre "Kurchatov Institute" - Institute of Macromolecular Compounds
A.V. Eremin
  • Affiliation: Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre "Kurchatov Institute" - Institute of Macromolecular Compounds
V.D. Krasikov
  • Affiliation: Branch of Petersburg Nuclear Physics Institute named by B.P.Konstantinov of National Research Centre "Kurchatov Institute" - Institute of Macromolecular Compounds
Volume/ Edition
Volume 80 / Issue number 11
Pages
1163-1174
Abstract
The features of planar (thin-layer) chromatography of several linear non-ionic polysaccharides (amylose, pullulan), their visualization by post-chromatographic derivatization with iodine, and the factors influencing this process are described. It is shown that the coloration of pullulan results from the formation of pseudoclathrate complexes of molecular iodine–iodide–pullulan in short helical segments of the polysaccharide, as well as iodine absorption in the bends of the polymer chain. It was established that the shift of the spectral absorption maximum of amylose (λ ~ 600 nm, blue coloration) to the shorter wavelength region (λ = 560 nm, red-brown coloration) is associated with specific interactions of starch with silica and the formation of a molecular iodine–iodide-anion–amylose–silica gel complex. It was demonstrated that the intensity of iodine staining of high-molecular-weight polysaccharides does not depend on their degree of polymerization, and polysaccharide visualization by the iodine reaction in thin-layer chromatography is possible only on neutral forms of silica sorbents.
Keywords
тонкослойная хроматография силикагель полисахариды пуллулан амилоза полийодид клатратные комплексы
Date of publication
01.11.2025
Year of publication
2025
Number of purchasers
0
Views
38

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