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RAS Chemistry & Material ScienceЖурнал аналитической химии Journal of Analytical Chemistry

  • ISSN (Print) 0044-4502
  • ISSN (Online) 3034-512X

CHROMATO-MASS SPECTROMETRIC DETERMINATION OF HYDROXYLATED POLYAROMATIC HYDROCARBONS IN URINEBY DISPERSION LIQUID-LIQUID MICROEXTRACTION AND DERIVATIZATION IN THE INJECTOR PORT

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PII
10.31857/S0044450224100086-1
DOI
10.31857/S0044450224100086
Publication type
Article
Status
Published
Authors
A. N. Alekseenko
  • Affiliation: East-Siberian Institute of Medical and Ecological Research
M. V. Kozlova
  • Affiliation: East-Siberian Institute of Medical and Ecological Research
Volume/ Edition
Volume 79 / Issue number 10
Pages
1132-1140
Abstract
A simple, rapid and sensitive method has been developed for the determination of hydroxylatedpolycyclic aromatic hydrocarbons (2-hydroxynaphthalene, 2-hydroxyfluorene, 9-hydroxyphenanthrene,3-hydroxyphenanthrene, 2-hydroxyphenanthrene, 1-hydroxypyrene, 6-hydroxychrizene) in urine by gaschromatography-mass spectrometry. Sample preparation is based on dispersion liquid-liquid microextraction with trichloromethane in an acidic medium. Derivatization is carried out in the heated port of theinjector with the reagent N,O-bistrimethylsilyltrifluoroacetamide. The type of dispersing solvent was experimentally selected, and using a three-factor experiment, the optimal ratio of the following factors wasestablished: pH values, volume of the dispersing solvent, and extraction time. For chromatography-massspectrometric analysis, a high-temperature NT-8 capillary column was used in the temperature gradient mode. The linearity range of the calibration function is 0.5-100 ng/ml. The developed method for thedetermination of hydroxylated PACs in urine has been tested on urine samples of aluminum production workers.
Keywords
гидроксилированные полиароматические углеводороды дисперсионная жидкостно-жидкостная микроэстракция силилирование в порту инжектора моча
Date of publication
15.09.2025
Year of publication
2025
Number of purchasers
0
Views
13

References

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