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RAS Chemistry & Material ScienceЖурнал аналитической химии Journal of Analytical Chemistry

  • ISSN (Print) 0044-4502
  • ISSN (Online) 3034-512X

DETERMINATION OF FORMALDEHYDE, GLYOXAL, GLUTARALDEHYDE, AND o-PHTHALALDEHYDE IN THE PRESENCE OF EACH OTHER IN DISINFECTANTS USING 2,4-DINTROPHENYLHYDRAZINE

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PII
S3034512X25120032-1
DOI
10.7868/S3034512X25120032
Publication type
Article
Status
Published
Authors
A.D. Salimova
  • Affiliation: Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
S.V. Andreev
  • Affiliation:
    Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
    Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian
L.Yu. Martynov
  • Affiliation: Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
A.A. Ischenco
  • Affiliation: Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University
R.D. Solovov
  • Affiliation:
    Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
    Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian
Volume/ Edition
Volume 80 / Issue number 12
Pages
1311-1318
Abstract
Dialdehydes are predominantly used as disinfectants because these compounds exhibit a broad spectrum of antimicrobial activity. Among monoaldehydes, formaldehyde is used to a limited extent due to its higher toxicity. This work describes conditions for the simultaneous derivatization of formaldehyde, glyoxal, glutaraldehyde, and o-phthalaldehyde with 2,4-dinitrophenylhydrazine. The reaction is carried out in an acetonitrile–methanol mixture at 50°C in an ultrasonic bath using trifluoroacetic acid as a catalyst. The best separation of mixture components was achieved on a C18 column in gradient elution mode with acetonitrile and acetate buffer solution (pH 5.4) at a variable flow rate. The linearity range for formaldehyde was 2.51–20.0 mg/L, for glutaraldehyde 4.92–21.9 mg/L, for o-phthalaldehyde 1.98–6.94 mg/L, and for glyoxal 2.00–10.0 mg/L. The limits of detection for formaldehyde, glyoxal, glutaraldehyde, and o-phthalaldehyde were 0.453, 0.177, 0.967, and 0.760 mg/L, respectively. The developed method was successfully applied for the simultaneous determination of aldehydes in disinfectants.
Keywords
2,4-динитрофенилгидразин формальдегид глиоксаль глутаровый альдегид ортофталевый альдегид дезинфицирующие средства
Date of publication
06.02.2026
Year of publication
2026
Number of purchasers
0
Views
99

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